Fig. 7.

²²Na⁺ transport by the Rnf complex reconstituted into proteoliposomes. 220 µl proteoliposomes (protein concentration 2.84 mg/ml) in buffer (100 mM Tris–HCl, 100 mM malic acid, pH 7.4, 5 mM MgCl₂, 2 mM NaCl, 2 mM DTE, and 4 μM resazurin) catalyzed ²²Na⁺ transport upon addition of 10 μl ferredoxin (3 mM), 10 μg CODH (3 mg/ml), and 30 μl NAD⁺ (100 mM) under anoxic conditions with CO in the headspace (■). One assay contained 40 µM of the protonophore TCS (▼). Another 40 µM of the Na⁺-ionophor ETH2120 (◆). One assay did not contain NAD⁺ (▲). Data points show one representative out of two biological replicates