Fig. 6
Ehrlich pathway engineering and cultivation optimization. a Screening of Ehrlich pathway mutations for enhanced 2-PE biosynthesis. b 2-phenylethanol (2-PE), 2-phenylethyl acetate (2-PEAc), phenylpyruvate (PP) and biomass (OD600) accumulation from d-glucose in fed-batch operation of the optimal strain, K. marxianus CBS 6556 eat1Δ his3Δ ura3Δ::(PKmTEF3)KmARO10, abz1::(PKmTEF3)KmARO4K221L-(PKmPGK)KmARO7G141S-(PKmTEF3)KmPHA2. Arrows indicated glucose addition. All experiments were performed in biological triplicates. Bars represent the mean, while error bars represent the standard deviation