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Table 1 Coded levels and real values of the three variables and responses in the FCCD experiment

From: Process optimization for mass production of 2,3-butanediol by Bacillus subtilis CS13

Run Sucrose (g/L) Ammonium citrate (g/L) MgSO4·7H2O (g/L) 2,3-BD (g/L)
1 0 (300) 0 (20) 0 (3) 124.66 ± 4.06
2 1 (350) − 1 (10) − 1 (1) 66.78 ± 2.03
3 − 1 (250) 1 (30) − 1 (1) 105.63 ± 3.42
4 − 1 (250) − 1 (10) − 1 (1) 93.10 ± 2.98
5 0 (300) 0 (20) 0 (3) 118.94 ± 3.36
6 0 (300) 1 (30) 0 (3) 115.10 ± 3.12
7 0 (300) 0 (20) 1 (5) 117.61 ± 3.24
8 0 (300) 0(20) 0 (3) 121.03 ± 3.95
9 1 (350) − 1 (10) 1 (5) 78.36 ± 2.76
10 0 (300) 0 (20) 0 (3) 124.81 ± 4.01
11 0 (300) − 1(10) 0 (3) 105.99 ± 3.13
12 1(350) 0 20) 0 (3) 95.44 ± 2.99
13 0 (300) 0 (20) 0 (3) 121.68 ± 4.03
14 0 (300) 0 (20) − 1 (1) 112.10 ± 3.53
15 − 1 (250) − 1(10) 1 (5) 107.05 ± 3.22
16 − 1 (250) 1 (30) 1 (5) 108.95 ± 3.22
17 1 (350) 1 (30) 1 (5) 94.41 ± 2.87
18 0 (300) 0 (20) 0 (3) 122.08 ± 4.03
19 − 1 (250) 0 (20) 0 (3) 116.76 ± 3.23
20 1 (350) 1 (30) − 1 (1) 82.99 ± 2.66