Fig. 5

C1-oxidized ends produced by 1 μM Cellulomonas LPMOs after 16 h on a panel of insoluble cellulose substrates. All substrates were assayed at 0.1% except NBSKP fiber which was assayed at 1% (no activity was detected at 0.1% NBSKP fiber). For each substrate, T. reesei cellulase cocktail (Celluclast; Sigma-Aldrich, P/N: C2730-30) was used to convert all C1-oxidized products into cellobionic acid and quantified as the total C1-oxidized ends generated (μM). Total oxidized ends were obtained by quantifying cellobionic acid by HPLC following hydrolysis of soluble products with T. reesei Celluclast enzyme cocktail. Each time point represents the average of three independent assays measured singly by HPLC, with error bars indicating the standard error of the mean