Table 2 Strains, genotype and plasmid used in the experimental work in this study

Shimwellia blattae

Type strain

CIP 104942, DSMZ 4481

Escherichia coli DH5α

endA1, hsdR17, supE44, thi-1 recA1, gyrA (Nal^R), relA1 Δ(argF-lac), U169 depR, Φ80dlacd(lacZ) M15

[44]

Lactococcus lactis IL1403

Type strain

Provided by Dr. P. López (CIB-CSIC)

pIZ1016

Gm^R, broad host range expression vector bearing lacI^q and Ptac. Rep (pBBR1MCS)

[46]

pIZ2

Extended pIZ1016 polylinker. EcoRI, ClaI, ScaI, SmaI (XmaI), SpeI, XvaI, SalI, PstI (SbfI), SphI, HindIII and SacI

Provided by Dr. G. Durante (CIB-CSIC)

pSEVA424

Sm^R/Sp^R, broad host range expression vector bearing lacI^q and Ptrc. Rep (RK2 replicative origin. oriV-trfA)

[47]

p424IbPSO

Sm^R/Sp^R. IbPSO operon (alsS-ilvC-ilvD-kdc) into pSEVA424

[22]

pIZadhA

Gm^R. adhA gene of L. lactis into pIZ2

This work

pIZpntAB

Gm^R. pntAB gene of E. coli into pIZ2

This work