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Table 2 Strains, genotype and plasmid used in the experimental work in this study

From: Modulating redox metabolism to improve isobutanol production in Shimwellia blattae

Strains Genotype References
Shimwellia blattae Type strain CIP 104942, DSMZ 4481
Escherichia coli DH5α endA1, hsdR17, supE44, thi-1 recA1, gyrA (NalR), relA1 Δ(argF-lac), U169 depR, Φ80dlacd(lacZ) M15 [44]
Lactococcus lactis IL1403 Type strain Provided by Dr. P. López (CIB-CSIC)
Plasmids Genotype References
pIZ1016 GmR, broad host range expression vector bearing lacIq and Ptac. Rep (pBBR1MCS) [46]
pIZ2 Extended pIZ1016 polylinker. EcoRI, ClaI, ScaI, SmaI (XmaI), SpeI, XvaI, SalI, PstI (SbfI), SphI, HindIII and SacI Provided by Dr. G. Durante (CIB-CSIC)
pSEVA424 SmR/SpR, broad host range expression vector bearing lacIq and Ptrc. Rep (RK2 replicative origin. oriV-trfA) [47]
p424IbPSO SmR/SpR. IbPSO operon (alsS-ilvC-ilvD-kdc) into pSEVA424 [22]
pIZadhA GmR. adhA gene of L. lactis into pIZ2 This work
pIZpntAB GmR. pntAB gene of E. coli into pIZ2 This work