Fig. 4
Expression of bfaA-B in S. cerevisiae. For all data the average and standard deviation of 3 independent experiments are shown. a Comparison of total dry cell weight (DCW) for wild-type (CEN.PK), Y&Z001 and MLM1.0 with empty vector (pRS416), low-copy bfaA-B (in pRS416) or high-copy bfaA-B (in pRS426). OD600 was measure after 72 h growth in minimal medium and converted using the measured constants for 1 OD for wild-type (0.687 ± 0.026), Y&Z001 (0.634 ± 0.017), and MLM1.0 (0.632 ± 0.047). b Comparison of total FFAs in the experiment shown in a was normalized to DCW. c Comparison of total BFA levels from the experiment in a normalized to the DCW. d Total FFAs and OD were measured for three S. cerevisiae strains expressing p416 bfaA-B grown in the absence or presence of 1.3 g/L methionine for 72 h in minimal medium. OD was converted to DCW using the same conversion constants as in a from. e Measurement of C16 and C18 BFAs from the experiment in d are shown. f Growth profile from the experiment shown in d and e