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Table 2 Acyl-chain substrate percent conversion of heterologous T. curvata bfa, M. hydrocarbonoclasticus tmp, and T. halophila tmp gene operons expressed in E. coli

From: Production of 10-methyl branched fatty acids in yeast

Exogenous fatty acid

T. curvata bfaAB

M. hydrocarbonoclasticus tmpBA

T. halophila tmpBA

12:1Δ11

–

–

–

13:1Δ12

–

–

–

14:1Δ9

3.4%

89%

95%

15:1Δ10

1.7%

86%

69%

16:1Δ9

30.4%

55%

95%

17:1Δ10

11.1%

36%

19%

18:1Δ6

–

–

–

18:1Δ9

33.7%

42%

47%

18:1Δ11

21.8%

9%

8%

18:1Δ9, 12-OH

–

–

–

18:1Δ9, 12

–

–

–

19:1Δ7

–

–

–

19:1Δ10

6.1%

–

–

20:1Δ5

–

–

–

20:1Δ8

–

–

–

20:1Δ11

2.2%

–

–

22:1Δ13

–

–

–

24:1Δ15

–

–

–

  1. Exogenous fatty acids were separately fed to Δcfa E. coli expressing the T. curvata bfa, M. hydrocarbonoclasticus tmp, and T. halophila tmp gene operons and a reference strain with a control vector. FAME profiles were compared across strains and to a control medium with no fatty acid supplementation. Conversion of the exogenously fed fatty acid was calculated as the combined percentage of 10-methyl and 10-methylene fatty acid peak areas relative to the sum of the unsaturated and branched fatty acid peak areas. –: not detected