Fig. 2

Comparison of lipid extraction from wet S. almeriensis after PEF-treatment using three different extraction systems, 24 h extraction with ethanol:hexane at ratios 1:0.41 vol/vol (a), 24 h extraction with pure ethanol (b) and 0.5 h extraction with ethanol at 60 °C (c). In the same graph, the effect of increasing the solvent volume by a factor 2 along with the effect of incubating the biomass for 24 h after PEF-treatment, are shown for systems A and B. In blocks, the average values of lipid yields from two independent experiments are presented along with the standard deviation as error bars. The straight grey line indicates the total lipid content as evaluated from extraction with the reference method, chloroform:methanol 2:1 vol/vol