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Table 1 Commonly used antibiotics and corresponding selectable markers

From: Approaches to genetic tool development for rapid domestication of non-model microorganisms

Antibiotic

Marker

Class

Kanamycin/neomycin

neo

Aminoglycoside

Chloramphenicol/thiamphenicol

cat

Chloramphenicol

Erythromycin

ermB, ermF

Macrolide

Ampicillin/carbenicillin

bla

Beta-lactam

Tetracycline

tetA

Tetracycline

Bleomycin

ble

Glycopeptide

Gentamicin

aacC1

Aminoglycoside

Streptomycin and spectinomycin

aadA

Aminoglycoside

Zeocin

zeo

Glycopeptide

Apramycin

apr

Aminoglycoside

Thiostrepton

tsr

Cyclic oligopeptide

Puromycin

pac

Aminonucleoside

Hygromycin

hph

Aminoglycoside

Nourseothricin

nat

Aminoglycoside

  1. Native antibiotic resistance levels can vary widely between strains. Therefore, an MIC experiment should be performed with each target organism to determine the level of antibiotic needed for selection.