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Table 2 Commonly used counter-selectable markers and associated selections for homologous recombination in bacteria

From: Approaches to genetic tool development for rapid domestication of non-model microorganisms

Marker

Counterselection

Reference

sacB

Sucrose

[101]

upp

5-Fluorouracil

[102]

hpt

8-Azahypoxanthine, others

[103]

tdk

5-Fluorodeoxyuridine

[104]

pyrF/ura3a

5-Fluoroorotic acid

[105]

pheS*(A294G)

p-Chloro-phenylalanine

[106]

codA

5-Fluorocytosine

[107]

Inducible mazF

N/A; expression is toxic

[95]

galK

2-Deoxygalactose

[108]

apt

2-Fluoroadenine

[109]

rpsL(strA)

Streptomycin

[110]

tetARa

Fusaric acid

[111]

thyA

Trimethoprim

[112]

Inducible ccdB

Expression is toxic

[113]

oroP

5-Fluoroorotate

[114]

pta-ack

Chloroacetate

[115]

  1. aCan be used as a positive selectable marker with uracil prototrophy and tetracycline resistance, respectively