Fig. 6

SDS-PAGE/Western immunoblot of the Synechocystis strains created in this study (Table 2) analysed for the presence of ethylene-forming enzyme (EFE). A SDS-PAGE loaded with 3 µg of protein crude extract from the Synechocystis engineered strain CD-P in different treatments (Table 3) and B Western immunoblot using anti-Flag antibody from the Synechocystis engineered strain CD-P in different treatments tested (Table 3). The different treatments were BG11, BG11₀, BG11+arginine, BG11₀+arginine, BG11+azaserine, BG11+cycloxydim, BG11₀+cycloxydim and BG11+arginine+azaserine+cycloxydim (Table 3) all containing 50 mM Tris pH 8.0, 50 mM NaHCO₃, Km (25 µg mL⁻¹), Cm (20 µg mL⁻¹), 5 µM of NiCl₂ and 20 µE m⁻² s⁻¹