Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

Table 3 Different treatments used in this study

Treatment	Media	Addition	Action	Effect on the cells
Standard	BG11	–
BG11₀	BG11₀	–	Nitrogen starvation	Higher substrate concentration: 2-oxoglutarate
BG11₀+Arg	BG11₀	Arginine	Nitrogen starvation and increased arginine	Higher substrates concentration: 2-oxoglutarate and arginine
BG11+Arg	BG11	Arginine	Increased arginine	Higher substrate concentration: arginine
BG11+Aza	BG11	Azaserine	Inhibition of the glutamine amino transferase in the glutamate synthase (GS-GOGAT cycle)	Higher substrate concentration: 2-oxoglutarate
BG11+Cyclo	BG11	Cycloxydim	Inhibition of acetyl-CoA carboxylase	Higher acetyl-CoA concentration
BG11₀+Cyclo	BG11₀	Cycloxydim	Nitrogen starvation and inhibition of acetyl-CoA carboxylase	Higher 2-oxoglutarate and acetyl-CoA concentration
BG11+Arg+ Aza+Cyclo	BG11	Arginine, azaserine and cycloxydim	Increased arginine, inhibition of the glutamine amino transferase in the glutamate synthase (GS-GOGAT cycle) and inhibition of acetyl-CoA carboxylase	Higher arginine, 2-oxoglutarate and acetyl-CoA concentration

In all treatments, 50 mM Tris pH 8.0, 50 mM NaHCO₃, Km (25 µg mL⁻¹), Cm (20 µg mL⁻¹) and 5 µM of NiCl₂ were added and the cells were incubated, 24 h under 20 µmol photons m⁻² s⁻¹

ISSN: 2731-3654