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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Metabolic engineering of Zymomonas mobilis for anaerobic isobutanol production

Fig. 3

Glucose (Glu) consumption, isobutanol (Iso) and ethanol (Eth) production of Z. mobilis strain ZM4-KT in RMG5 with different concentrations of tetracycline. The codon-optimized kdcA gene from L. lactis driven by the Ptet inducible promoter was cloned into the shuttle vector pEZ15Asp, which was then confirmed and transformed into Z. mobilis strain ZM4 to generate the recombinant strain ZM4-KT. Tc 0, Tc 1 and Tc 2 represented the tetracycline concentrations of 0, 0.2, and 1.0 μg/mL that was added into the medium one time at the beginning of the experiment, respectively. At least two independent experiments were carried out with similar results. Values are the mean of one representative experiment with three technical replicates. Error bars represent standard deviations

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