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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Metabolic engineering of Zymomonas mobilis for anaerobic isobutanol production

Fig. 5

Plasmid constructs to divert carbon flux from ethanol production to isobutanol biosynthesis and their performance in recombinant strains. Six plasmids (pEZ-B, pEZ-A0, pEZ-A1, pEZ-A2, pEZ-A3, and pEZ-A4) containing different combinations of four als genes from different bacteria (ZmAls from Z. mobilis, EcAls from E. cloacae, BlAls from Bacillus licheniformis, and BsAls from B. subtilis) as well as ilvC and ilvD genes from Z. mobilis for isobutanol production (a). Ethanol (Eth) and isobutanol (Iso) production in the recombinant Z. mobilis strains ZMQ1-B, ZMQ1-A0, ZMQ1-A1, ZMQ1-A2, ZMQ1-A3, and ZMQ1-A4 after 24 h post-induction in RMG5 with different tetracycline inducer concentrations (b). Tc 0, Tc 0.2 and Tc 1 represented the tetracycline concentrations of 0, 0.2, and 1.0 μg/mL that was added into the medium one time at the beginning of the experiment, respectively. At least two independent experiments were carried out with similar results. Values are the mean of one representative experiment with three or more technical replicates. Error bars represent standard deviations

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