Fig. 4From: Improved bioethanol productivity through gas flow rate-driven self-cycling fermentationAnalysis of samples from SCF demonstration. Cycle time (a) amount of biomass (b), and concentrations of glucose (c) and ethanol (d) were plotted for the beginning and end of all cycles. Medium supplemented with ergosterol (0.02 g/L) and Tween 80 (0.8 g/L) was used for cycles 1–17; the concentrations of ergosterol and Tween 80 were tripled for the remaining cycles. Cycle 4 was disrupted by halting agitation and temperature control for ~ 7 h. Cycle numbers were labeled at the initial stage of each cycle, with the exception of a where the cycle number was indicated on the x-axis. The horizontal line in a represents the fermentation time for batch operated under similar conditions (Additional file 4: Table S1). In b–d data are reported as the average from at least three analytical replicates, with error bars representing standard deviationsBack to article page