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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Improved bioethanol productivity through gas flow rate-driven self-cycling fermentation

Fig. 5

Online monitoring parameters from SCF demonstration. Gas flow rate (a), slope of gas flow rate (b), total volume of gas evolved per cycle (c), and pH (d) were monitored throughout SCF operation. Medium supplemented with ergosterol (0.02 g/L) and Tween 80 (0.8 g/L) was used for cycles 1–17; the concentrations of ergosterol and Tween 80 were tripled for the remaining cycles. Cycle 4 was disrupted by halting agitation and temperature control for ~ 7 h. Cycle numbers were labeled at the top of each figure, with the exception of c where the cycle numbers were indicated on the x-axis. The horizontal line in c represents the total volume of gas evolved in batch operation conducted under similar conditions (Additional file 5: Figure S4c)

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