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Table 1 Comparison of fatty acid methyl ester (FAME) extraction methods for biodiesel production from Chlamydomonas sp. KNM0029C

From: Co-production of biodiesel and bioethanol using psychrophilic microalga Chlamydomonas sp. KNM0029C isolated from Arctic sea ice

MethodPretreatmentLipid extractionCatalyst; conditionSolventReferences
LewisFreeze-drying biomass (100 mg)Methanol/chloroform (10:1)HCl; 90 °C, 2 hHexane/chloroform (1:1)[22]
Bligh & DyerFreeze-drying biomass (100 mg)Methanol/chloroform (2:1)HCl; 90 °C, 2 hChloroform[23]
SasserFreeze-drying biomass (100 mg)Methanol/hexane/methyl tert-butyl ether (2:1:1)HCl: 80 °C, 10 minHexane/methyl tert-butyl ether (1:1)[24]
Modified FAME extraction AWet biomass (100 mg DCW), sonication treatmentMethanol/chloroform (2:1)HCl; 90 °C, 1 hChloroformThis study
Modified FAME extraction BWet biomass (100 mg DCW), sonication treatmentMethanol/chloroform (10:1)HCl; 90 °C, 1 hHexane/chloroform (1:1)This study
  1. DCW dry cell weight