Table 1 Comparison of fatty acid methyl ester (FAME) extraction methods for biodiesel production from Chlamydomonas sp. KNM0029C
Method | Pretreatment | Lipid extraction | Catalyst; condition | Solvent | References |
|---|---|---|---|---|---|
Lewis | Freeze-drying biomass (100 mg) | Methanol/chloroform (10:1) | HCl; 90 °C, 2 h | Hexane/chloroform (1:1) | [22] |
Bligh & Dyer | Freeze-drying biomass (100 mg) | Methanol/chloroform (2:1) | HCl; 90 °C, 2 h | Chloroform | [23] |
Sasser | Freeze-drying biomass (100 mg) | Methanol/hexane/methyl tert-butyl ether (2:1:1) | HCl: 80 °C, 10 min | Hexane/methyl tert-butyl ether (1:1) | [24] |
Modified FAME extraction A | Wet biomass (100 mg DCW), sonication treatment | Methanol/chloroform (2:1) | HCl; 90 °C, 1 h | Chloroform | This study |
Modified FAME extraction B | Wet biomass (100 mg DCW), sonication treatment | Methanol/chloroform (10:1) | HCl; 90 °C, 1 h | Hexane/chloroform (1:1) | This study |