Fig. 8From: Metabolic engineering of the cellulolytic thermophilic fungus Myceliophthora thermophila to produce ethanol from cellobioseConstruction and characterization of JY518Pyc strain. a Strategy for construction of JY518Pyc via homologous recombination. b Confirmation of JY518Pyc by PCR. PCR1 and PCR2 were performed with the primers 2295692QC-F/2295692QC-R. The PCR product of JY518Pyc is 6.4 kb (Lane 1 and 2) while that of WT is 4.9 kb. c Fermentation profile of JY518Pyc strainBack to article page