Fig. 2

Phylogenetic placement and ploidy determination of C. intermedia. a Phylogenetic tree of C. intermedia CBS 141442 and PYCC 4715 and 15 other ascomycetous yeasts. The molecular phylogenetic analysis was based on ten protein-encoding genes using the maximum likelihood method. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The numbers at each branch indicate bootstrap values, and the CTG branch is highlighted. b Ploidy determination of C. intermedia CBS 141442 by flow cytometry. Three S. cerevisiae strains were used as reference; haploid BY4741, diploid CEN.PK and tetraploid 122 MDS