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Table 1 N-glycosylation motifs of CTendo45 and its mutants

From: Improvement of the catalytic activity and thermostability of a hyperthermostable endoglucanase by optimizing N-glycosylation sites

Enzyme

Motif

Modification

Existing sites

CTendo45

N88-E89-T90

–

N88

L47T

N45-A46-T47

Addition

N45 and N88

Q56T

N54-A55-T56

Addition

N54 and N88

G63T

N61-D62-T63

Addition

N61 and N88

R67S

N65-T66-S67

Addition

N65 and N88

T90A

N88-E89-A90

Deletion

–

F143T

N141-H142-T143

Addition

N141 and N88

F143S

N141-H142-S143

Addition

N141 and N88

W169S

N167-G168-S169

Addition

N167 and N88

R67S/T90A

N65-T66-S67

Substitution

N65

N88-E89-A90

  
  1. Glycans linked to the marked Asn residues were modified in mutants