Substrate | Enzyme | Activity (IU/mg) | Fold |
---|---|---|---|
CMC-Na | CTendo45 | 1.21 ± 0.08 | 1.00 |
L47T | 1.36 ± 0.06 | 1.12 | |
Q56T | 1.28 ± 0.10 | 1.06 | |
G63T | 0.91 ± 0.08 | 0.75 | |
R67S | 1.58 ± 0.12 | 1.31 | |
T90A | 2.24 ± 0.18 | 1.85 | |
F143T | 0.20 ± 0.04 | 0.17 | |
F143S | 0.16 ± 0.02 | 0.13 | |
W169S | 0.07 ± 0.01 | 0.06 | |
R67S/T90A | 2.73 ± 0.15 | 2.26 | |
β-d-glucan | CTendo45 | 2.07 ± 0.14 | 1.00 |
L47T | 2.17 ± 0.08 | 1.05 | |
Q56T | 2.09 ± 0.15 | 1.01 | |
G63T | 1.71 ± 0.11 | 0.83 | |
R67S | 2.62 ± 0.13 | 1.27 | |
T90A | 3.39 ± 0.21 | 1.64 | |
F143T | 0.98 ± 0.04 | 0.47 | |
F143S | 0.87 ± 0.04 | 0.42 | |
W169S | 0.25 ± 0.06 | 0.12 | |
R67S/T90A | 4.01 ± 0.28 | 1.94 |