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Table 2 Activities of CTendo45 and its mutants on CMC-Na and β-d-glucan as substrates

From: Improvement of the catalytic activity and thermostability of a hyperthermostable endoglucanase by optimizing N-glycosylation sites

Substrate

Enzyme

Activity (IU/mg)

Fold

CMC-Na

CTendo45

1.21 ± 0.08

1.00

L47T

1.36 ± 0.06

1.12

Q56T

1.28 ± 0.10

1.06

G63T

0.91 ± 0.08

0.75

R67S

1.58 ± 0.12

1.31

T90A

2.24 ± 0.18

1.85

F143T

0.20 ± 0.04

0.17

F143S

0.16 ± 0.02

0.13

W169S

0.07 ± 0.01

0.06

R67S/T90A

2.73 ± 0.15

2.26

β-d-glucan

CTendo45

2.07 ± 0.14

1.00

L47T

2.17 ± 0.08

1.05

Q56T

2.09 ± 0.15

1.01

G63T

1.71 ± 0.11

0.83

R67S

2.62 ± 0.13

1.27

T90A

3.39 ± 0.21

1.64

F143T

0.98 ± 0.04

0.47

F143S

0.87 ± 0.04

0.42

W169S

0.25 ± 0.06

0.12

R67S/T90A

4.01 ± 0.28

1.94

  1. Values are mean ± SD of three replicates