Fig. 4

Construction of a platform strain for recombinant enzyme production by deleting pyrG and ligD of Aspergillus oryzae strain AOK27L. a Schematic of pyrG deletion. The resulting strain was named A. oryzae strain HO1. b PCR assay confirming pyrG deletion. The primer set used and the target sizes of amplicons with and without pyrG are shown in a. cA. oryzae strain HO1 on a CD agar plate with and without uridine, showing uridine auxotrophy. d Schematic of ligD deletion. The resulting strain was named A. oryzae strain HO2. e PCR assay confirming ligD gene deletion. The primer set used and the target sizes of amplicons with and without ligD are shown in d. fA. oryzae strain HO2 on a CD agar plate with and without uridine, showing uridine auxotrophy. PCR polymerase chain reaction