Fig. 5
From: Feasibility study of on-site solid-state enzyme production by Aspergillus oryzae
Heterologous saccharification enzyme production using Aspergillus oryzae strain HO2. a Introduction of the enzyme production cassette into the original pyrG locus. b SDS-PAGE for liquid cultures of A. oryzae strain HO2 and the three transformed strains possessing three respective genes: EX from Talaromyces aurantiacus (calculated molecular weight: 34 kDa), BGL from T. cellulolyticus (83 kDa), and CBH from T. cellulolyticus (52 kDa). SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; EX endoxylanase, BGL β-glucosidase, CBH cellobiohydrolase