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Table 3 Frequency of homologous integration before and after ligD deletion

From: Feasibility study of on-site solid-state enzyme production by Aspergillus oryzae

Strain (ligD gene)

Obtained transformants

Homologous integrants

Gene-targeting efficiency (%)

HO1 (+)

36

4

11

HO2 (−)

39

38

97

  1. For strain HO1, data were obtained from the integration of the ligD deletion cassette (Fig. 4d). For strain HO2, data were obtained from the integration of the CBH production cassette (Fig. 5a)
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