Table 5 PCR templates and primers used to prepare transformation inserts
From: Feasibility study of on-site solid-state enzyme production by Aspergillus oryzae
Primer pair | Sequence (5′ to 3′) | Template |
|---|---|---|
PyrG or ligD deletion cassettes | ||
 pRI | gcaattaatgtgagttagctcactc | pRIdP or pRIdLR |
ggatatcggggaagaacagtatgtc | ||
Enzyme production cassettes | ||
 pMD20c | cagtgagcgcaacgcaattaatgtgagttag | pPPO enzyme |
gggatgtgctgcaaggcgattaagttg | ||