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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Whole-cell biocatalysis for hydrogen storage and syngas conversion to formate using a thermophilic acetogen

Fig. 2

Effect of ionophores/uncoupling agents and pH on product formation by resting cells of T. kivui. Cells were grown with 0.1 M pyruvate, harvested in the end-exponential growth phase and suspended in buffer (50 mM Imidazole, 20 mM KCl, 20 mM MgSO4, 2 mM DTE, 4 µM Resazurin, pH 7.0 or 25 mM MES, 25 mM MOPS, 25 mM HEPES, 25 mM EPPS, 25 mM CHES, 20 mM KCl, 20 mM MgSO4, 2 mM DTE, 4 µM Resazurin, pH as indicated) to a final concentration of 1 mg/mL. a Cells were incubated without or b with ionophores/uncoupling agents or (c) at the corresponding pH for 10 min at 60 °C. The experiment was started by replacing the gas phase with H2 + CO2 (80:20%, 2 × 105 Pa) and the product formation was determined after 40 (b) or 90 min (c). Squares and white bars, acetic acid; triangles and grey bars, formic acid

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