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Table 5 A comparison of intracellular metabolites in CRZ14e and CRZ14e-ED

From: Coexistence of the Entner–Doudoroff and Embden–Meyerhof–Parnas pathways enhances glucose consumption of ethanol-producing Corynebacterium glutamicum

Metabolite Intracellular metabolite (mM) Ratio P valuea
CRZ14e CRZ14e-ED
G6P 0.22 ± 0.01 0.25 ± 0.02 1.1 0.04
F6P 0.03 ± 0.00 0.03 ± 0.01 1.0 0.27
FBP 34 ± 3 38 ± 2 1.1 0.12
DHAP 3.4 ± 0.1 3.7 ± 0.2 1.1 0.03
GAP 0.16 ± 0.01 0.23 ± 0.03 1.4 0.02
BPG 0.04 ± 0.02 0.08 ± 0.01 2.0 0.02
3PG 0.72 ± 0.08 1.15 ± 0.06 1.6 0.00
PEP 0.12 ± 0.00 0.17 ± 0.02 1.4 0.02
PYR 2.18 ± 0.41 2.29 ± 0.13 1.1 0.68
Ru5P 0.19 ± 0.01 0.12 ± 0.01 0.6 0.00
R5P 0.09 ± 0.01 0.07 ± 0.00 0.8 0.02
6PG 0.03 ± 0.00 0.09 ± 0.01 3.0 0.00
ADP 0.37 ± 0.02 0.32 ± 0.03 0.9 0.07
ATP 1.70 ± 0.05 1.92 ± 0.10 1.1 0.02
NAD 0.28 ± 0.03 0.37 ± 0.07 1.3 0.14
NADH 0.07 ± 0.00 0.07 ± 0.02 1.0 0.97
NADP 0.17 ± 0.02 0.11 ± 0.01 0.6 0.01
NADPH 0.02 ± 0.01 0.10 ± 0.01 5.0 0.00
KDPG 0.00 ± 0.00 0.09 ± 0.01 NA 0.00
ATP/ADP 4.64 ± 0.29 5.98 ± 0.49 1.3 0.02
NADH/NAD 0.24 ± 0.02 0.19 ± 0.09 0.8 0.45
  1. Data obtained from 2-h reactions show the average and the standard deviation from three measurements
  2. aStudent’s t test was used for statistical analyses