Fig. 7

AMPK complex involves in regulation of glucose dual-affinity transport system. a Glucose uptake of WT, Δprk-10, and Δrco-3 mutants in low glucose concentration. **P < 0.01. b Relative expression levels of glt-1 in WT, Δprk-10, Δsnf4, Δrco-3, Δrco-3;Δprk-10, and Δrco-3;Δsnf4 strains of N. crassa in glucose and no-carbon conditions. c Relative expression levels of hgt-1 in WT, Δprk-10, Δsnf4, Δrco-3, Δrco-3;Δprk-10, and Δrco-3;Δsnf4 strains in glucose and no-carbon conditions. Mycelia were grown in VMM supplemented with 2% sucrose for 16 h, then transferred to VMM with or without 2% glucose. After additional cultivation for 1 h, mycelia were harvested and the glt-1 expression level was determined by qRT-PCR