Skip to main content
Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae

Fig. 3

Time course of fed-batch fermentation of EC∆budC∆ldhA. a Glucose, acetoin, succinic acid, b by-product. The experiments were conducted in 50 mL of fermentation medium containing 60 g L−1 glucose in a 250 mL flask. NaHCO3 (1 g L−1) was added at 6 and 12 h of fermentation. After that, NaHCO3 (2 g L−1) was added at 24, 36, 48, 61, 72, and 84 h of fermentation. Glucose (20 g L−1) was added at 24, 36, 48, 61, 72, and 84 h of fermentation. Samples were withdrawn every 12 h for detection of cell density and concentration of substrates and products

Back to article page