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Table 3 Strains and plasmid used in this study

From: Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae

Name Relevant genotype Source and ref
Strains
 DH5α Fφ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk,mk+) supE44λ thi -1 gyrA96 relA1 phoA, Recipient in transformations Sangon Biotech
 S17-1 λpir RP4-2(Km::Tn7,Tc::Mu-1) pro-82 LAMpir recA1 endA1 thiE1 hsdR17 creC510; conjugative strain able to host -pir-dependent plasmids Zoman Biotechnology
 E. cloacae CICC 10011 Wild type CICC
 EC∆budC E. cloacae CICC 10011 budC disruption mutant strain This study
 EC∆ldhA E. cloacae CICC 10011 ldhA disruption mutant strain This study
 EC∆budC∆ldhA E. cloacae CICC 10011 budC and ldhA disruption mutant strain This study
Plasmid
 pGEM-T Easy Vector Cloning vector, Apr Promega
 pToriR6K pGEM-T Easy Vector with 0.6-kb oriR6K fragment, Apr This study
 pTBCup pGEM-T Easy Vector with 0.5-kb budC upstream fragment, Apr This study
 pTBCdown pGEM-T Easy Vector with 0.5-kb budC downstream fragment, Apr This study
 pTLAup pGEM-T Easy Vector with 0.5-kb ldhA upstream fragment, Apr This study
 pTLAdown pGEM-T Easy Vector with 0.5-kb ldhA downstream fragment, Apr This study
 pK18mobSacB oriT(RP4) sacB lacZα Plac Pmbi; mobilization and counterselection, Kanr BCRC
 pRL27 Mini-Tn5 transposon (oriR6K) delivery vector, Kanr [33]
 pKR6K R6K replicon; gene replacement vector, Kanr This study
 pKΔbudC pKR6K derivative, carries a 771 bp deletion of budC, Kanr This study
 pK∆ldhA pKR6K derivative, carries a 990 bp deletion of ldhA, Kanr This study
  1. CICC, The China Center of Industrial Culture Collection, China; BCRC, The Bioresource Collection and Research Centre, FIRDI, Taiwan