From: Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae
Name | Relevant genotype | Source and ref |
---|---|---|
Strains | ||
DH5α | F−φ80 lac ZΔM15 Δ(lacZYA-arg F) U169 endA1 recA1 hsdR17(rk−,mk+) supE44λ− thi -1 gyrA96 relA1 phoA, Recipient in transformations | Sangon Biotech |
S17-1 λpir | RP4-2(Km::Tn7,Tc::Mu-1) pro-82 LAMpir recA1 endA1 thiE1 hsdR17 creC510; conjugative strain able to host -pir-dependent plasmids | Zoman Biotechnology |
E. cloacae CICC 10011 | Wild type | CICC |
EC∆budC | E. cloacae CICC 10011 budC disruption mutant strain | This study |
EC∆ldhA | E. cloacae CICC 10011 ldhA disruption mutant strain | This study |
EC∆budC∆ldhA | E. cloacae CICC 10011 budC and ldhA disruption mutant strain | This study |
Plasmid | ||
pGEM-T Easy Vector | Cloning vector, Apr | Promega |
pToriR6K | pGEM-T Easy Vector with 0.6-kb oriR6K fragment, Apr | This study |
pTBCup | pGEM-T Easy Vector with 0.5-kb budC upstream fragment, Apr | This study |
pTBCdown | pGEM-T Easy Vector with 0.5-kb budC downstream fragment, Apr | This study |
pTLAup | pGEM-T Easy Vector with 0.5-kb ldhA upstream fragment, Apr | This study |
pTLAdown | pGEM-T Easy Vector with 0.5-kb ldhA downstream fragment, Apr | This study |
pK18mobSacB | oriT(RP4) sacB lacZα Plac Pmbi; mobilization and counterselection, Kanr | BCRC |
pRL27 | Mini-Tn5 transposon (oriR6K) delivery vector, Kanr | [33] |
pKR6K | R6K replicon; gene replacement vector, Kanr | This study |
pKΔbudC | pKR6K derivative, carries a 771 bp deletion of budC, Kanr | This study |
pK∆ldhA | pKR6K derivative, carries a 990 bp deletion of ldhA, Kanr | This study |