Fig. 2

Catalytic activity of AA10_07. a MALDI-TOF MS analysis of products released by 1 µM AA10_07 from 1% (w/v) Avicel in the presence of 1 mM ascorbic acid. The figure shows signals representing LPMO products within the DP4–DP7 range (DP, degree of polymerization). Oxidized and native products are observed as sodium adducts and/or as sodium adducts of sodium salts, as indicated (Glc, glucose; Glc1A, gluconic acid; GlcLA, lactone). Annotated product peaks were not detected in negative control reactions lacking the enzyme or lacking the reductant. b A chromatographic analysis of oxidized products generated by 1 µM AA10_07 from 1% (w/v) Avicel. The picture includes a chromatogram of a product mixture that had been treated with TfCel6A and a chromatogram showing the GlcGlc1A (DP2ox) standard