Fig. 4

Cellulose solubilisation by AA10_07 in the presence of ascorbic acid or gallic acid. The figure shows the release of oxidised products in LPMO reactions (1 µM AA10_07 in 50 mM sodium phosphate buffer, pH 6.0, 30 °C) with 1% (w/v) Avicel that were carried out in the absence of hydrogen peroxide (a) or in the presence of 200 µM H₂O₂ (b) using 1 mM ascorbic acid (circular markers) or 1 mM gallic acid (triangle markers) as a reductant. Error bars indicate standard deviations between triplicates. Note that a, b feature different time scales. Product accumulation was not observed in control reactions with substrate and reductant or in reactions with substrate, reductant and H₂O₂