Fig. 7

Avicel solubilisation by increasing amounts of AA10_07 in the presence of ascorbic acid or gallic acid. The figure shows the release of oxidised products from 1% (w/v) Avicel in 9 h reactions with 0.01–8 µM AA10_07 in 50 mM sodium phosphate buffer, pH 6.0, 30 °C. The experiments were carried out using 1 mM ascorbic acid (dashed line) or 1 mM gallic acid (solid line) as a reductant. Error bars indicate standard deviations between triplicates. Note that the plotted data are derived from LPMO progress curves shown in Additional file 1: Fig. S3. All these reactions displayed linear behavior, except for experiments with 0.01 µM or 0.025 µM enzyme and gallic acid