Fig. 8

The effect of free copper on AA10_07 reactions with cellulose in the presence of ascorbic acid or gallic acid. The figure shows progress curves obtained for AA10_07 reactions (1 µM LPMO in 50 mM sodium phosphate buffer, pH 6.0, 30 °C) with 1% (w/v) Avicel using 1 mM ascorbic acid (a) or 1 mM gallic acid (b) and various concentrations of free copper. Note that b also features the progress curve obtained with 3 µM free copper and 1 mM ascorbic acid, which is shown in a, for reference. Error bars indicate standard deviations between triplicates. Note the different time scale compared to Fig. 4a. Product accumulation was not observed in control reactions with no enzyme, containing substrate, free copper, and reductant