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Table 1 Cellulose oxidation rates derived from previously reported ScLPMO10C reactions driven by ascorbic acid and molecular oxygen

From: Unraveling the roles of the reductant and free copper ions in LPMO kinetics

Approximate cellulose oxidation rate

Product quantification routinec

Substrate and reaction conditions

References

0.2 min−1 a

DP2ox quantification (HPAEC-PAD) after treatment with TrCel7A cellobiohydrolase

0.2% (w/v) PASC, 20 mM ammonium acetate buffer, pH 6.0, 40 °C, 2 mM ascorbic acid

[24]: Fig. 2a

0.14 min−1 a

DP2ox quantification (HPAEC-PAD) after treatment with TfCel5A endogluconase

1% (w/v) Avicel, 50 mM sodium phosphate buffer, pH 6.0, 40 °C, 1 mM ascorbic acid

[41]: Fig. 2

6.7 min−1

DP2ox + DP3ox quantification (HPAEC-PAD) after treatment with TfCel5A endogluconase

1% (w/v) Avicel, 50 mM sodium phosphate buffer, pH 7.0, 40 °C, 1 mM ascorbic acid

[42]: Fig. 4

3.4 min−1 b

DP2ox + DP3ox quantification (HPAEC-PAD) after treatment with TfCel5A endogluconase

1% (w/v) Avicel, 50 mM sodium phosphate buffer, pH 7.0, 40 °C, 1 mM ascorbic acid

[12]: Fig. 1f

2.5 min−1 b

DP2ox + DP3ox quantification (HPAEC-PAD) after treatment with TfCel5A endogluconase

0.5% (w/v) PASC, 50 mM sodium phosphate buffer, pH 6.0, 40 °C, 1 mM ascorbic acid

[43]: Fig. 8

7.9 min−1

DP2ox + DP3ox quantification (HPAEC-PAD) after treatment with TfCel5A endoglucanase

1% (w/v) Avicel, 50 mM sodium phosphate buffer, pH 7.0, 40 °C, 1 mM ascorbic acid

[20]: Fig. 2a

  1. aThe reported rate is underestimated by approximately twofold due to limitations in product quantification. Only C1-oxidized cellobiose was quantified in the mixture of C1-oxidized cellobiose and C1-oxidized cellotriose. The molar ratio between these products is typically close to 1 in reactions with ScLPMO10C [41]
  2. bNo progress curves were reported in the paper meaning that approximate oxidation rates were estimated using single time points
  3. cDP2ox/DP3ox: C1-oxidized cellobiose/cellotriose