From: Unraveling the roles of the reductant and free copper ions in LPMO kinetics
Approximate cellulose oxidation rate | Product quantification routinec | Substrate and reaction conditions | References |
---|---|---|---|
0.2 min−1 a | DP2ox quantification (HPAEC-PAD) after treatment with TrCel7A cellobiohydrolase | 0.2% (w/v) PASC, 20 mM ammonium acetate buffer, pH 6.0, 40 °C, 2 mM ascorbic acid | [24]: Fig. 2a |
0.14 min−1 a | DP2ox quantification (HPAEC-PAD) after treatment with TfCel5A endogluconase | 1% (w/v) Avicel, 50 mM sodium phosphate buffer, pH 6.0, 40 °C, 1 mM ascorbic acid | [41]: Fig. 2 |
6.7 min−1 | DP2ox + DP3ox quantification (HPAEC-PAD) after treatment with TfCel5A endogluconase | 1% (w/v) Avicel, 50 mM sodium phosphate buffer, pH 7.0, 40 °C, 1 mM ascorbic acid | [42]: Fig. 4 |
3.4 min−1 b | DP2ox + DP3ox quantification (HPAEC-PAD) after treatment with TfCel5A endogluconase | 1% (w/v) Avicel, 50 mM sodium phosphate buffer, pH 7.0, 40 °C, 1 mM ascorbic acid | [12]: Fig. 1f |
2.5 min−1 b | DP2ox + DP3ox quantification (HPAEC-PAD) after treatment with TfCel5A endogluconase | 0.5% (w/v) PASC, 50 mM sodium phosphate buffer, pH 6.0, 40 °C, 1 mM ascorbic acid | [43]: Fig. 8 |
7.9 min−1 | DP2ox + DP3ox quantification (HPAEC-PAD) after treatment with TfCel5A endoglucanase | 1% (w/v) Avicel, 50 mM sodium phosphate buffer, pH 7.0, 40 °C, 1 mM ascorbic acid | [20]: Fig. 2a |