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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: CRISPRi screens reveal genes modulating yeast growth in lignocellulose hydrolysate

Fig. 2

CRISPRi screens are reproducible and capture positive controls. a Schematic of screen procedure and selection conditions. All selections are performed with populations grown with ATc to induce CRISPRi and without ATc as reference, and in biological triplicate cultures. b MDS-plot of read count samples, depicting Euclidian distance variation in two dimensions (x- and y-axis) to estimate (dis)similarity of replicate samples and effect size between CRISPRi-induced (+ ATc) and reference samples (-ATc) across all screen conditions. The 2-dimensional MDS-plot was generated with the default edgeR function to illustrate similarity between samples. c Correlation of guide log2FCs from this study to a previous screen from Jann et al. [34]. CRISPRi effects of both screens report on fitness in SC medium. Jann et al. [34] phenotyped the TF and PK libraries separately with two replicates while in the presented screens we phenotyped a single consisting of a combined TF and PK libraries and measured triplicates. d Distribution of log2 gene fold changes for non-essential (blue) and essential genes (gold)

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