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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: CRISPRi screens reveal genes modulating yeast growth in lignocellulose hydrolysate

Fig. 3

Genes with specific functions in hydrolysate fitness. a Scatter of gene log2FCs in SCM versus SCM + 10% hydrolysate conditions. Dots denote essential (brown) and non-essential genes (green). Genes with strong hydrolysate-specific effect on fitness are labelled. b Dilution spot plating of CRISPRi strains grown with or without 250 ng/µL ATc for 24 h and plated on SCM or SCM + 10% hydrolysate agar plates. The no gRNA control strain (noGuideCtr) harbours a non-functional gRNA. The HOG1 repression strain (HOG1_rep) expresses a HOG1-targeting gRNA. c Venn diagram of genes modulating fitness in SCM, SCM + 10% hydrolysate and SCM + 45% inhibitor cocktail conditions. The overlap of significant genes is shown together with volcano plots to illustrate perturbation strength and confidence of individual hits. Volcano plot of gene log2FCs and -log10 Benjamini–Hochberg FDRs for CRISPRi effects in d SCM, e SCM + 10% hydrolysate, and f in SCM + 45% inhibitor cocktail. The dashed blue line marks an FDR of 0.05. Some but not all significant modulators are labelled for clarity. g Guide RNA log2FCs for selected genes across conditions. Dots denote gRNA log2FCs and are coloured by FDR for single genes (not for non-essential and essential gene panels). Diamonds denote the means and are coloured in green for SCM, blue for SCM + 10% hydrolysate and yellow for SCM + 45% inhibitor compound conditions akin to colours used in df. For genes, the mean gRNA log2FCs (diamonds) correspond to their gene log2FC

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