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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Blocking drug efflux mechanisms facilitate genome engineering process in hypercellulolytic fungus, Penicillium funiculosum NCIM1228

Fig. 7

Functional analysis of Δcbh1 strain secretome. a SDS-PAGE gel of secretome of NCIM1228 (+ ve control) and four Δcbh1 mutants showing band corresponding to CBH1 protein missing in Δcbh1 secretome; second panel showing Western blot performed with anti-CBH1 antibody confirming the absence of CBH1. b Activities for Avicelase c CMCase, xylanase and β-glucosidase were measured in the secretome of NCIM1228 and ΔCBH1 when grown in modified RCM cellulase production medium. d Glucose and e xylose released after time intervals of 12 h, 24 h and 48 h of saccharification of pretreated rice straw (5%) by the NCIM1228 and ΔCBH1 secretome (30 mg/g of dry biomass weight)

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