Fig. 5

Ume6p mediates the effect of HYP2 overexpression on yeast cells response to acetic acid. a Direct targets of Ume6p involved in acetic acid response. The log2 values of relative transcription levels were compared. (1) Genes involved in chromatin remodeling, transcription and RNA processing. (2) Genes involved in protein modification, trafficking and sorting. (3) Genes involved in mitochondrial function. (4) Genes involved in carbohydrate metabolism. (5) Gene involved in remodeling and maintaining cell wall architecture. (6) Unknown function. b Transcription levels of Ume6p direct targets. c Effect of Ume6p activity on cell growth upon acetic acid stress. d Effect of Ume6p activity on transcription of BEM4, BUD21 and IME4. Yeast cells were cultured under conditions with or without 87 mM acetic acid (pH 4.2) for 4 h. The relative transcription of target gene in YS58-V under non-stressed condition was defined as a value of 1, respectively. Data are presented as the means of the results of three independent experiments. Error bars represent standard deviations (*P < 0.05, **P < 0.01)