Fig. 5

Effects of glucose on protein production, respiration, and sugar uptake of PC-3-7 and PC-3-7ΔtubB. a Secretory protein production, d the concentration of CO₂ in the exhaust gas and f glucose and cellobiose concentration when cultivated in medium with 10% (w/v) microcrystalline cellulose. b Secretory protein production, e the concentration of CO₂ in the exhaust gas and g glucose and cellobiose concentration when cultivated in medium with 10% (w/v) microcrystalline cellulose and 2.5% (w/v) glucose. Blue lines indicate PC-3-7 and orange lines indicate PC-3-7ΔtubB. c Cellulolytic and hemicellulolytic enzyme activities in the culture supernatant after 120 h of cultivation, including pNPGase (the BGL activity), pNPLase (the CBH1 activity), CMCase (the EG activity), pNPXase (the BXL activity), pNPX2ase and xylanase (the XYN activity). In f and g, solid lines indicate glucose and dashed lines indicate cellobiose. Data are expressed as mean ± SD of three biological replicates. For secretory protein production, statistical significance was determined by two-tailed unpaired Student’s t-test. **p < 0.01. The arrows in c and e indicate the time at which cells were collected for RNA-seq analysis