Fig. 3

Determination of transgene expression by transcriptional (qRT-PCR) and western blot (WB) analysis. a The ferritin transcript level of FerIN transgenic switchgrass. b The ferritin transcript level of FerIN/IBPex transgenics. c The IBP transcript level of FerIN/IBPex transgenics. d WB analysis of ferritin expression in FerIN transgenics. e WB analysis of IBP and ferritin expression in FerIN/IBPex transgenics. For qRT-PCR, the transgenics with the lowest expression level of ferritin or IBP was set to 1. Data are presented as the mean (± standard error, SE) of three replicates and bars represented by different letters are significantly different at p < 0.05 between lines analyzed by one-way ANOVA with Tukey’s test. For WB, the corresponding antibodies used are indicated on the right side of the WB result