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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees

Fig. 6

Overexpression of PttVAP27-17 affected only slightly the wood chemistry of field-grown trees. af Py–GC/MS analysis of cell wall components in WT and four PttVAP27-17 lines (1–4). The values are relative, and depict the combined area of GC peaks assigned to carbohydrates (C), lignin (L), syringyl lignin (S-lignin), guaiacyl lignin (G-lignin) and p-hydroxyphenyl lignin (H-lignin) as a percentage of total GC peak area. S/G, ratio between syringyl and guaiacyl-type lignin. gl Total carbohydrate content for WT and PttVAP27-17 lines 1–4 determined by high-performance anion-exchange chromatography. The fractions of arabinose, galactose, glucose, xylose, mannose, and total monosaccharides (arabinose + galactose + glucose + xylose + mannose) released after acid hydrolysis are indicated as percentages (g of monosaccharide sugar in anhydrous form per 100 g of dry weight of wood). For all analyses, mature wood was collected from the base of the stem of 5-year-old trees grown in the field. n = 3 for transgenic line 1, n = 5 for transgenic line 2, 3, 4 and n = 8 for the WT where “n” indicates number of biological replicates. The box plots show the median (horizontal line) with the outer limits at the 25th and 75th percentiles, the 1.5 inter-quartile ranges (whiskers) and the outliers (gray spots). Asterisks indicate significant differences from the WT at p ≤ 5% (*) according to Student’s t-test. Percentages indicate increase (+) or decrease (−) in values for lines overexpressing PttVAP27-17 compared to the WT

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