Fig. 7

The impact of the overexpression of PttVAP27-17 on the enzymatic saccharification in woody tissues of field-grown trees. a, b Glucose production rate (GPR) after 2 h of enzymatic saccharification (gL⁻¹ h⁻¹), for samples without (untreated) and with acid pretreatment (pretreated) in wild type and four transgenic lines (1–4). c, d Glucose yields after 72 h of enzymatic saccharification [g glucose per g of wood (dry weight) without (untreated) and with acid pretreatment (pretreated)]. e Total wood glucose yield (TWG) after pretreatment and 72 h of enzymatic saccharification per whole tree stem biomass. TWG was calculated by using the formula 1/3 × π × radius² × height × wood density × glucose yield after pretreatment and 72 h of enzymatic saccharification. n = 3 for transgenic line 1, n = 5 for transgenic line 2, 3, 4 and n = 8 for the WT; where “n” indicates number of biological replicates. Mature wood was collected from the base of the stem of 5-year-old trees grown in the field. The box plots show the median (horizontal line) with the outer limits at the 25th and 75th percentiles, the 1.5 inter-quartile ranges (whiskers) and the outliers (gray spots). Asterisks indicate significant differences from the WT at p ≤ 5% (*), according to Student’s t-test. Percentages indicate increase (+) or decrease (−) in values for lines overexpressing PttVAP27-17 compared to the WT