Fig. 1
From: Increased expression of fatty acid and ABC transporters enhances seed oil production in camelina
Overexpression of AtFAX1 and AtABCA9 in camelina. a The overexpression (OE) constructs of AtFAX1 and AtABCA9. b Immunoblotting of HA-tagged FAX1 and Flag-tagged ABCA9 in AtFAX1-OE, AtABCA9-OE, and AtFAX1/ABCA9-OE camelina leaves. Total proteins (10 µg/lane) were extracted from leaves of 3-week-old plants, separated by 10% SDS-PAGE, and transferred to a polyvinylidene difluoride membrane. The membrane was blotted with anti-HA or anti-Flag antibody conjugated with alkaline phosphatase. Lanes OE1 through OE10 represent different transgenic lines harboring the FAX1-HA or ABCA9-Flag OE construct. Numbers on the left of each panel mark protein molecular mass standards in kilodaltons. c The phylogenetic tree of FAX1 (left) and ABCA9 (right) in camelina, and their expression pattern in seedlings, leaves, roots, stems, and 2- and 4-week (W) old developing seeds. ACT2 (actin2) was used as the internal standard for cDNA input adjustment. The expression level is relative to the value of Csa04g043700.1 for FAX1 and Csa02g069700.1 for ABCA9 in seedlings (red dashed line). Values are means ± SE (n = 3)