Acetate as substrate for l-malic acid production with Aspergillus oryzae DSM 1863

Table 2 Comparison of malic acid production with A. oryzae and different substrate concentrations after 192 h

Substrate	c (substrate) (g/L)	Consumed substrate (g/L)	c (malic acid) (g/L)	Y_P/S (g/g)	Y_{P/S, carbon} (g/g)	Overall Productivity (g/(L*h))	Maximum productivity (g/(L*h))	Time of maximum productivity (h)
HAc	10	9.72 ± 0.15	0.00 ± 0.00	0.00	0.00	0.000	0.000	0.00
	20	18.43 ± 1.54	1.42 ± 0.34	0.08	0.07	0.007	0.020	107.15
	30	29.64 ± 0.06	4.56 ± 0.23	0.15	0.14	0.024	0.076	92.25
	40	38.01 ± 1.74	7.04 ± 0.33	0.19	0.17	0.037	0.123	89.61
	45	44.14 ± 0.53	8.44 ± 0.42	0.19	0.17	0.044	0.122	85.05
	50	42.58 ± 0.80	8.45 ± 0.49	0.20	0.18	0.044	0.115	90.33
	55	37.55 ± 2.27	7.92 ± 0.29	0.21	0.19	0.041	0.081	95.38
Mixed	HAc: 45 glucose: 5	HAc: 44.87 ± 0.30 glucose: 5.06 ± 0.04	9.95 ± 0.25	0.20	0.18	0.052	0.114	61.98
	HAc: 45 glucose: 10	HAc: 44.46 ± 0.30 glucose: 10.07 ± 0.03	12.15 ± 0.21	0.22	0.20	0.063	0.119	58.62
	HAc: 45 glucose: 15	HAc: 44.66 ± 0.33 glucose: 15.06 ± 0.04	14.18 ± 0.09	0.24	0.21	0.074	0.138	61.02
Glucose^a	109	58.90 ± 0.86	38.75 ± 0.29	0.66	0.59	0.231	0.292	87.61

^a The values for 109 g/L glucose were determined for a cultivation time of 168 h
HAc acetic acid, Y_P/S (g/g) substrate specific malic acid yield calculated as g(malic acid)/g(total consumed substrate(s)), Y_{P/S, carbon} (g/g) substrate specific malic acid yield calculated as g(produced C from malic acid)/g(total consumed C from substrate(s))

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