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Table 3 Organic acid distribution for cultivation of A. oryzae with different substrate concentrations after 192 h

From: Acetate as substrate for l-malic acid production with Aspergillus oryzae DSM 1863

Substrate c (substrate) (g/L) c (total acids) (g/L) Organic acid distribution (%)
Malate Succinate Fumarate Pyruvate α-Ketoglutarate Oxalate Citrate
HAc 10 0.58 ± 0.14 0.0 0.0 1.4 0.0 0.0 98.6 0.0
  20 3.95 ± 0.54 35.6 23.3 2.5 0.6 0.9 37.1 0.0
  30 8.37 ± 0.20 54.5 35.0 3.4 0.6 0.6 5.8 0.0
  40 12.69 ± 0.82 55.5 37.1 4.1 0.6 0.5 1.2 1.0
  45 14.86 ± 0.77 56.8 35.9 4.2 0.8 0.4 0.8 1.1
  50 15.11 ± 0.92 55.9 37.1 4.2 0.6 0.5 0.7 1.0
  55 14.32 ± 0.32 55.3 37.1 4.7 0.6 0.4 0.9 0.9
Mixed HAc: 45 glucose: 5 20.54 ± 0.42 48.5 41.5 5.3 2.7 0.3 0.4 1.4
  HAc: 45 glucose: 10 25.28 ± 0.39 48.1 39.3 5.9 4.8 0.3 0.4 1.2
  HAc: 45 glucose: 15 29.75 ± 0.23 47.7 39.7 5.9 4.9 0.3 0.2 1.3
Glucosea 109 51.88 ± 1.15 74.7 14.6 1.5 0.4 0.3 0.3 8.1
  1. a The values for 109 g/L glucose were determined for a cultivation time of 168 h
  2. HAc   acetic acid; percentages were calculated based on the acid concentration in g/L as fraction of the total acid concentration in g/L