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Table 3 Organic acid distribution for cultivation of A. oryzae with different substrate concentrations after 192 h

From: Acetate as substrate for l-malic acid production with Aspergillus oryzae DSM 1863

Substrate

c (substrate) (g/L)

c (total acids) (g/L)

Organic acid distribution (%)

Malate

Succinate

Fumarate

Pyruvate

α-Ketoglutarate

Oxalate

Citrate

HAc

10

0.58 ± 0.14

0.0

0.0

1.4

0.0

0.0

98.6

0.0

 

20

3.95 ± 0.54

35.6

23.3

2.5

0.6

0.9

37.1

0.0

 

30

8.37 ± 0.20

54.5

35.0

3.4

0.6

0.6

5.8

0.0

 

40

12.69 ± 0.82

55.5

37.1

4.1

0.6

0.5

1.2

1.0

 

45

14.86 ± 0.77

56.8

35.9

4.2

0.8

0.4

0.8

1.1

 

50

15.11 ± 0.92

55.9

37.1

4.2

0.6

0.5

0.7

1.0

 

55

14.32 ± 0.32

55.3

37.1

4.7

0.6

0.4

0.9

0.9

Mixed

HAc: 45 glucose: 5

20.54 ± 0.42

48.5

41.5

5.3

2.7

0.3

0.4

1.4

 

HAc: 45 glucose: 10

25.28 ± 0.39

48.1

39.3

5.9

4.8

0.3

0.4

1.2

 

HAc: 45 glucose: 15

29.75 ± 0.23

47.7

39.7

5.9

4.9

0.3

0.2

1.3

Glucosea

109

51.88 ± 1.15

74.7

14.6

1.5

0.4

0.3

0.3

8.1

  1. a The values for 109 g/L glucose were determined for a cultivation time of 168 h
  2. HAc   acetic acid; percentages were calculated based on the acid concentration in g/L as fraction of the total acid concentration in g/L