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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Colorimetric LPMO assay with direct implication for cellulolytic activity

Fig. 2

Effect of DHA on the formation of PHP as measured by absorption at 552 nm: a Progress of the rPHP assay was followed by stopping the reaction with 200 mM Na2CO3 pH 10.3 at various times (0–120 min) using the spectrophotometers build in injector module. rPHP was oxidised by 0.3 µM Cu-TaAA9 (orange), supplemented with 100 µM DHA (blue) or 100 µM ascorbate (green). rPHP oxidation by 0.3 µM CuCl2 supplemented with 100 µM DHA is shown for comparison (red). DHA appears to enhance the oxidation of rPHP. b Comparison of the DHA-enhancing effect on rPHP oxidation for 12 different proteins. LPMOs, were loaded with CuCl2 and 1 µM of each enzyme was incubated for 30 min at 40 °C with or without DHA. The enhancing effect of DHA is shown in black and the absorbance without DHA is shown in white bars

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