Fig. 4

Activity profiles of TaAA9A with various co-substrates. a The rPHP oxidation temperature profiles using potential boosting compounds, 100 µM GSH (orange), 100 µM DHA (red), 1.25 mM fructose (purple), Milli-Q water (green), and ascorbate (blue). b Cellulose degradation products analysed by HPAEC after incubation of 0.75 µM TaAA9A with 0.4% PASC in 25 mM citrate phosphate buffer at pH 7.25 and 50 °C. The co-substrates were added in 1 mM concentration and the samples were incubated for 1 h, except for the sample with fructose, which was incubated for 23 h. Chromatograms are representative of duplicate experiments and colour coded as in (a). Glucose and fructose monomers have retention times of about 5 min. Peaks at 12–20 min are from not-oxidised sugars, peaks at 20–25 min are from C1-oxidised sugars, while peaks at 28 min are from two-times oxidised sugar products