Fig. 5

Activity of TaAA9A with ascorbate, DHA, O₂ and H₂O₂. a rPHP oxidation assay with various co-substrates: Milli-Q water (black), 100 µM ascorbate (blue), 100 µM DHA (red) in combinations with or without 100 µM H₂O₂. The sample with “no O₂” was prepared, incubated and stopped inside a strictly anaerobic glovebox. b, c Cellulose degradation analysed by HPAEC after 23 h incubation using ascorbate (blue) or DHA (red) as co-substrates. Samples were as described in Fig. 4, but prepared in double volumes and inside a strictly anaerobic glovebox. Samples were then split in two, one portion was removed from the glovebox and incubated in normal atmosphere b, the other portion incubated in the anaerobic environment c