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Table 1 Release of glucose, productivity, and degree of conversion of raw cassava starch to glucose from raw cassava starch hydrolyses by StargenTM002 at various enzyme dosages, temperatures, and initial pH values

From: Single-step ethanol production from raw cassava starch using a combination of raw starch hydrolysis and fermentation, scale-up from 5-L laboratory and 200-L pilot plant to 3000-L industrial fermenters

Main factors

Glucose concentration (g/L)

Productivity, rp (g/L/h)

Degree of conversion to glucose (%w/w)

StargenTM002 (% v/w)

   

 0.1

37.45 ± 5.98c

0.99 ± 0.27c

17.02 ± 2.72c

 0.2

56.56 ± 1.36b

1.18 ± 0.03b

25.71 ± 0.62b

 0.3

73.78 ± 4.18a

1.54 ± 0.09a

33.54 ± 1.90a

 0.4

78.70 ± 1.41a

1.64 ± 0.03a

35.77 ± 0.64a

Temperature (°C)

   

 30

30.07 ± 0.39c

0.63 ± 0.01c

13.67 ± 0.18c

 35

59.50 ± 5.31b

1.24 ± 0.11b

27.05 ± 2.41b

 40

133.27 ± 7.83a

2.78 ± 0.16a

60.58 ± 3.56a

Initial pH

   

 3.0

114.39 ± 1.24a

2.38 ± 0.03a

52.00 ± 0.56a

 4.0

96.39 ± 1.03b

2.01 ± 0.02b

43.82 ± 0.47b

 5.0

56.26 ± 1.17c

1.17 ± 0.02c

25.57 ± 0.53c

 6.0

33.10 ± 1.14d

0.69 ± 0.02d

15.04 ± 0.52d

 7.0

16.59 ± 0.36e

0.35 ± 0.01e

7.70 ± 0.16e

  1. Statistic comparisons of those mean values within their own columns (among main factors) at p-values ≤ 0.05 show different characters, a, b, c, d, and e, which indicate statically significant differences